The Impact of Differing Types of Physical Activity on Weight Loss, Strength, VO2 max, and the Various Metabolic Hormones.

PURPOSE: The purpose of this investigate the impact of differing types of physical activity on weight loss, strength, VO2 max, and various metabolic hormones. METHODS: Eleven sedentary and overweight male/females participated in this study and were randomly assigned to one of two exercise groups: aerobic training (A) (N = 4, 40 ± 8.7 yrs 165 ± 8.3cm, 89 ± 16 kg), or aerobic training plus resistance training (ART) (N = 7, 43 ± 10 yrs, 171 ± 8.7 cm, 91 ± 12 kg). Training protocols for the two exercise groups consisted of 30 min of aerobic exercise five days per week at 60-70% of heart rate max and 30 minutes of aerobic exercise three days per week at 60-70% of heart rate max plus two days of moderate intensity resistance exercise per week for the (A) and (ART) groups, respectively. Baseline testing consisted of weight, hip, and waist measurements, and body composition analysis using bioelectrical impedance. Participants donated approximately 20 ml of fasting blood for the analysis of clinical chemistry profiles, WBC counts, and the hormones insulin, leptin, and cortisol. Following these assessments, VO2 max, upper-body strength, and lower-body strength was established using standard procedures. All baseline assessments were repeated at 4 and 8 weeks of the study. Statistical analyses utilized a two-way ANOVA with repeated measures for all criterion variables (p\u3c0.05). RESULTS: A significant (p \u3c 0.05) increase in upper and lower body strength was observed in both A and ART groups, however there was no significant difference between groups. A significant group x time interaction (p \u3c 0.05) was observed for body weight (ART: -1.5 ± 0.3 lbs; A: 3 ± 0.5 lbs). No significant (p \u3e 0.05) main effects for group or time were observed for the criterion variables body fat, VO2 max, and the hormones insulin, leptin, and cortisol. Furthermore, neither protocol had a significant impact on body fat or VO2 max. CONCLUSIONS: It can be concluded that aerobic exercise coupled with resistance training for 8 weeks yielded greater results in weight loss than aerobic training alone. No marked changes were noted on the affects of any type of exercise regiment on body composition, VO2 max, or the hormones leptin, insulin, and cortisol over the 8-week study

Normal Aging Modulates the Neurotoxicity of Mutant Huntingtin

Aging likely plays a role in neurodegenerative disorders. In Huntington's disease (HD), a disorder caused by an abnormal expansion of a polyglutamine tract in the protein huntingtin (Htt), the role of aging is unclear. For a given tract length, the probability of disease onset increases with age. There are mainly two hypotheses that could explain adult onset in HD: Either mutant Htt progressively produces cumulative defects over time or “normal” aging renders neurons more vulnerable to mutant Htt toxicity. In the present study, we directly explored whether aging affected the toxicity of mutant Htt in vivo. We studied the impact of aging on the effects produced by overexpression of an N-terminal fragment of mutant Htt, of wild-type Htt or of a β-Galactosidase (β-Gal) reporter gene in the rat striatum. Stereotaxic injections of lentiviral vectors were performed simultaneously in young (3 week) and old (15 month) rats. Histological evaluation at different time points after infection demonstrated that the expression of mutant Htt led to pathological changes that were more severe in old rats, including an increase in the number of small Htt-containing aggregates in the neuropil, a greater loss of DARPP-32 immunoreactivity and striatal neurons as assessed by unbiased stereological counts

Automatic Text Simplification of News Articles in the Context of Public Broadcasting

This report summarizes the work carried out by the authors during the Twelfth Montreal Industrial Problem Solving Workshop, held at Universit\'e de Montr\'eal in August 2022. The team tackled a problem submitted by CBC/Radio-Canada on the theme of Automatic Text Simplification (ATS)

Loss of the thyroid hormone-binding protein Crym renders striatal neurons more vulnerable to mutant huntingtin in Huntington's disease

The mechanisms underlying preferential atrophy of the striatum in Huntington's disease (HD) are unknown. One hypothesis is that a set of gene products preferentially expressed in the striatum could determine the particular vulnerability of this brain region to mutant huntingtin (mHtt). Here, we studied the striatal protein µ-crystallin (Crym). Crym is the NADPH-dependent p38 cytosolic T3-binding protein (p38CTBP), a key regulator of thyroid hormone (TH) T3 (3,5,3′-triiodo-l-thyronine) transportation. It has been also recently identified as the enzyme that reduces the sulfur-containing cyclic ketimines, which are potential neurotransmitters. Here, we confirm the preferential expression of the Crym protein in the rodent and macaque striatum. Crym expression was found to be higher in the macaque caudate than in the putamen. Expression of Crym was reduced in the BACHD and Knock-in 140CAG mouse models of HD before onset of striatal atrophy. We show that overexpression of Crym in striatal medium-size spiny neurons using a lentiviral-based strategy in mice is neuroprotective against the neurotoxicity of an N-terminal fragment of mHtt in vivo. Thus, reduction of Crym expression in HD could render striatal neurons more susceptible to mHtt suggesting that Crym may be a key determinant of the vulnerability of the striatum. In addition our work points to Crym as a potential molecular link between striatal degeneration and the THs deregulation reported in HD patient